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| Ribeiro,Luciana A.; Azevedo,João L.; Aragão,Francisco J.L.; Rech,Elibio L.; Schmidt,Gilberto S.; Coutinho,Luiz L.. |
| Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed <FONT FACE="Symbol">b</FONT>-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos. |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010 |
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| Elias,Luciana M.; Fortkamp,Diana; Sartori,Sérgio B.; Ferreira,Marília C.; Gomes,Luiz H.; Azevedo,João L.; Montoya,Quimi V.; Rodrigues,André; Ferreira,Antonio G.; Lira,Simone P.. |
| ABSTRACT Anthracnose is a crop disease usually caused by fungi in the genus Colletotrichum or Gloeosporium. These are considered one of the main pathogens, causing significant economic losses, such as in peppers and guarana. The current forms of control include the use of resistant cultivars, sanitary pruning and fungicides. However, even with the use of some methods of controlling these cultures, the crops are not free of anthracnose. Additionally, excessive application of fungicides increases the resistance of pathogens to agrochemicals and cause harm to human health and the environment. In order to find natural antifungal agents against guarana anthracnose, endophytic fungi were isolated from Amazon guarana. The compounds piliformic acid and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2-Hexylidene-3-methylbutanedioic acid; Amazon; Anthracnose; Plant pathogen. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400840 |
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